Coding

Part:BBa_K4226002

Designed by: Siyu Han   Group: iGEM22_CAFA_China   (2022-09-19)


hrpS Gene

This part is an improvement for the part：HrpS Gene uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of hrpL Promoter. The Amp30E Amplification Device consists of hrpR Gene, hrpS and hrpL Promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 902
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 112
    Illegal SapI.rc site found at 745


Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.

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Categories
Parameters
None